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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene continues to be observed in many tumors, which results in chromosome instability, and so may perhaps lead to tumorigenesis. This protein was identified to associate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a significant position in marketing cell-cycle development within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, happen to be explained. Two extra splice variants were being documented but comprehensive nucleotide sequence information and facts is just not yet readily available.
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Blocking peptides are peptides that bind precisely for the focus on antibody and block antibody binding. These peptide typically has the epitope recognized with the antibody.
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This tutorial outlines even more achievable optimization of cellular and immunoassay parameters to be certain the very best final results are...
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This guidebook outlines even more attainable optimization of mobile and immunoassay parameters to be sure the very best effects are...
Antibodies sure to the blocking peptide no more bind to your epitope to the focus on protein. By evaluating the staining from your blocked antibody compared to the antibody on your own, you can see which staining is distinct.
Focus on Details Cyclin E belongs for the extremely conserved cyclin family members, whose members are characterised by a spectacular periodicity in protein abundance throughout the cell cycle. Cyclins functionality as regulators of CDK kinases. Distinctive cyclins show unique expression and degradation patterns which add on the temporal coordination of each mitotic celebration. Cyclin E types a posh with and features for a regulatory subunit of CDK2, whose activity is needed for mobile cycle G1/S transition. This protein accumulates for the G1-S stage boundary and is degraded as cells development by means of S section.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by the two antibodies...
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